Primers Available
Organism: Manihot esculenta chloroplast
Repeat:(AT)6
Start:130330 End:130341
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCGTTGATTCGTTTCTAGATCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCAGTCGGTTCAGTATGG | |||||||||||||
| Tm_F_1 | 57.422 | Size_F_1 | 23 | Tm_R_1 | 59.751 | Size_R_1 | 20 | PRODUCT Size | 208 bp | Primer Start | 130158 | Primer End | 130365 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCGTTGATTCGTTTCTAGATCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCAGTCGGTTCAGTATGGT | |||||||||||||
| Tm_F_2 | 57.422 | Size_F_2 | 23 | Tm_R_2 | 59.751 | Size_R_2 | 20 | PRODUCT Size | 207 bp | Primer Start | 130158 | Primer End | 130364 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGTGCCGCCCTTATCAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTTGTATGAATCGTTATTGGTTCG | |||||||||||||
| Tm_F_3 | 59.961 | Size_F_3 | 20 | Tm_R_3 | 59.098 | Size_R_3 | 25 | PRODUCT Size | 104 bp | Primer Start | 130297 | Primer End | 130400 |