Primers Available
Organism: Manihot esculenta chloroplast
Repeat:(A)14
Start:161219 End:161232
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGGGTTATCCTGCACTTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCGGGACCACGTTACTATT | |||||||||||||
| Tm_F_1 | 59.669 | Size_F_1 | 20 | Tm_R_1 | 59.174 | Size_R_1 | 20 | PRODUCT Size | 254 bp | Primer Start | 161101 | Primer End | 161354 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTTGGGGTTATCCTGCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCGGGACCACGTTACTATT | |||||||||||||
| Tm_F_2 | 60.396 | Size_F_2 | 20 | Tm_R_2 | 59.174 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 161097 | Primer End | 161354 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGGTTATCCTGCACTTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAATGGTAGATGCCCGGGAC | |||||||||||||
| Tm_F_3 | 59.669 | Size_F_3 | 20 | Tm_R_3 | 58.947 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 161101 | Primer End | 161366 |