Primers Available
Organism: Manihot esculenta chloroplast
Repeat:(A)13
Start:120272 End:120284
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTCCAGATCAATGCCAAATTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTTCACCATAGCGGCTTGC | |||||||||||||
| Tm_F_1 | 58.177 | Size_F_1 | 23 | Tm_R_1 | 59.259 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 120150 | Primer End | 120429 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGATATGTCCAGATCAATGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGGCTTGCTCGAAATCATA | |||||||||||||
| Tm_F_2 | 57.29 | Size_F_2 | 22 | Tm_R_2 | 59.063 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 120145 | Primer End | 120418 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTAACACAAATTCTCTCCACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTGCTTCCTAAGAGCAGCGT | |||||||||||||
| Tm_F_3 | 57.439 | Size_F_3 | 23 | Tm_R_3 | 60.108 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 120182 | Primer End | 120457 |