Primers Available
Organism:Polytomella capuana mitochondrion
Repeat:(GCTGTT)3
Start:3548 End:3565
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTTTTGGCTGAATCCGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCAAAATCGCCGTAAACAC | |||||||||||||
| Tm_F_1 | 59.551 | Size_F_1 | 20 | Tm_R_1 | 59.867 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 3525 | Primer End | 3801 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTTTTGGCTGAATCCGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAATCGCCGTAAACACCATC | |||||||||||||
| Tm_F_2 | 59.551 | Size_F_2 | 20 | Tm_R_2 | 59.829 | Size_R_2 | 20 | PRODUCT Size | 273 bp | Primer Start | 3525 | Primer End | 3797 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAACGTGAGTTTTTGGCTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGACAAACAGCGGGATTTGA | |||||||||||||
| Tm_F_3 | 59.881 | Size_F_3 | 20 | Tm_R_3 | 60.636 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 3517 | Primer End | 3773 |