Primers Available
Organism: Microhyla okinavensis mitochondrion
Repeat:(AAT)4
Start:8488 End:8499
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCTGGTTTCAAGCCAGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAATAGGGGAGGCTGCGTCT | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 60.106 | Size_R_1 | 20 | PRODUCT Size | 165 bp | Primer Start | 8429 | Primer End | 8593 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTGGTTTCAAGCCAGACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAATAGGGGAGGCTGCGTCT | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 60.106 | Size_R_2 | 20 | PRODUCT Size | 164 bp | Primer Start | 8430 | Primer End | 8593 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGAATCGAACCCCCTTACCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAATAGGGGAGGCTGCGTCT | |||||||||||||
| Tm_F_3 | 59.818 | Size_F_3 | 20 | Tm_R_3 | 60.106 | Size_R_3 | 20 | PRODUCT Size | 181 bp | Primer Start | 8413 | Primer End | 8593 |