Primers Available
Organism: Iotrochota birotulata mitochondrion
Repeat:(TTTAT)3
Start:13535 End:13549
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTACATGTGGGGGAATAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCACCACCCGTACCCTTTA | |||||||||||||
| Tm_F_1 | 59.926 | Size_F_1 | 21 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 210 bp | Primer Start | 13392 | Primer End | 13601 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTACATGTGGGGGAATAGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCTAGACCCACCCCTCCAC | |||||||||||||
| Tm_F_2 | 59.926 | Size_F_2 | 21 | Tm_R_2 | 60.253 | Size_R_2 | 20 | PRODUCT Size | 225 bp | Primer Start | 13392 | Primer End | 13616 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACATGTGGGGGAATAGCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCACCACCCGTACCCTTTA | |||||||||||||
| Tm_F_3 | 58.691 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 13395 | Primer End | 13601 |