Primers Available
Organism: Callyspongia plicifera mitochondrion
Repeat:(TTA)4
Start:3589 End:3600
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGCTTTTGTGGAACAAGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCACCGGCTAATACTGGCA | |||||||||||||
| Tm_F_1 | 59.968 | Size_F_1 | 20 | Tm_R_1 | 60.153 | Size_R_1 | 19 | PRODUCT Size | 271 bp | Primer Start | 3352 | Primer End | 3622 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATTCTGGGGGATCTGTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCACCGGCTAATACTGGCA | |||||||||||||
| Tm_F_2 | 59.816 | Size_F_2 | 20 | Tm_R_2 | 60.153 | Size_R_2 | 19 | PRODUCT Size | 204 bp | Primer Start | 3419 | Primer End | 3622 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTTTGTGGAACAAGGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCACCGGCTAATACTGGCA | |||||||||||||
| Tm_F_3 | 60.25 | Size_F_3 | 20 | Tm_R_3 | 60.153 | Size_R_3 | 19 | PRODUCT Size | 269 bp | Primer Start | 3354 | Primer End | 3622 |