Primers Available
Organism: Callyspongia plicifera mitochondrion
Repeat:(ATT)4(TTAT)3*
Start:16740 End:16758
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GATGGAGGCCGAATGGTTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCCCAATCATAGCAACCA | |||||||||||||
| Tm_F_1 | 60.107 | Size_F_1 | 20 | Tm_R_1 | 59.369 | Size_R_1 | 20 | PRODUCT Size | 135 bp | Primer Start | 16663 | Primer End | 16797 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGATGGAGGCCGAATGGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCCCCAATCATAGCAACCA | |||||||||||||
| Tm_F_2 | 60.107 | Size_F_2 | 20 | Tm_R_2 | 59.369 | Size_R_2 | 20 | PRODUCT Size | 136 bp | Primer Start | 16662 | Primer End | 16797 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAGGCCGAATGGTTCAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCCCCAATCATAGCAACCA | |||||||||||||
| Tm_F_3 | 59.669 | Size_F_3 | 20 | Tm_R_3 | 59.369 | Size_R_3 | 20 | PRODUCT Size | 133 bp | Primer Start | 16665 | Primer End | 16797 |