Primers Available
Organism: Puntius tetrazona mitochondrion
Repeat:(ATAAA)3
Start:1144 End:1158
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTGAGCCAAACAGCTAGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTTGCTTGGGATTACTGGCT | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 59.958 | Size_R_1 | 22 | PRODUCT Size | 253 bp | Primer Start | 1095 | Primer End | 1347 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACCCTGAGCCAAACAGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTTGCTTGGGATTACTGGCT | |||||||||||||
| Tm_F_2 | 59.963 | Size_F_2 | 20 | Tm_R_2 | 59.958 | Size_R_2 | 22 | PRODUCT Size | 256 bp | Primer Start | 1092 | Primer End | 1347 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTGAGCCAAACAGCTAGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGTTTCGGGGTTTCAAACT | |||||||||||||
| Tm_F_3 | 60.036 | Size_F_3 | 20 | Tm_R_3 | 57.853 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 1095 | Primer End | 1374 |