Primers Available
Organism: Bufo japonicus mitochondrion
Repeat:(TA)12
Start:2273 End:2296
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCTCAGTCAACGCGCATAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCTAAGACCAGGGGAAGA | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.582 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 2144 | Primer End | 2388 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCTCAGTCAACGCGCATAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCTAAGACCAGGGGAAGAC | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.458 | Size_R_2 | 20 | PRODUCT Size | 244 bp | Primer Start | 2144 | Primer End | 2387 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAACCCTGTCCATCGACCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCCTAAGACCAGGGGAAGA | |||||||||||||
| Tm_F_3 | 59.675 | Size_F_3 | 20 | Tm_R_3 | 59.582 | Size_R_3 | 20 | PRODUCT Size | 193 bp | Primer Start | 2196 | Primer End | 2388 |