Primers Available
Organism: Diodon holocanthus mitochondrion
Repeat:(CTCC)3
Start:5780 End:5791
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTCCTAATCCGAGCCGAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCTTCTACGCCTGAGGAAG | |||||||||||||
| Tm_F_1 | 59.968 | Size_F_1 | 20 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 5568 | Primer End | 5826 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAATAATCGGCGCCCCAGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCTTCTACGCCTGAGGAAG | |||||||||||||
| Tm_F_2 | 59.966 | Size_F_2 | 20 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 107 bp | Primer Start | 5720 | Primer End | 5826 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTAATAATCGGCGCCCCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCTTCTACGCCTGAGGAAG | |||||||||||||
| Tm_F_3 | 59.965 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 109 bp | Primer Start | 5718 | Primer End | 5826 |