Primers Available
Organism: Diodon holocanthus mitochondrion
Repeat:(ACAA)3
Start:13479 End:13490
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTGTCGCCCTTGAACTTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTGCATCGCTTGTTGTGG | |||||||||||||
| Tm_F_1 | 60.04 | Size_F_1 | 20 | Tm_R_1 | 59.969 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 13451 | Primer End | 13702 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTGTCGCCCTTGAACTTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGTGATGGCCTTTGGTCCA | |||||||||||||
| Tm_F_2 | 60.04 | Size_F_2 | 20 | Tm_R_2 | 59.962 | Size_R_2 | 20 | PRODUCT Size | 211 bp | Primer Start | 13451 | Primer End | 13661 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTGTCGCCCTTGAACTTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTGATGGCCTTTGGTCCAG | |||||||||||||
| Tm_F_3 | 60.04 | Size_F_3 | 20 | Tm_R_3 | 59.961 | Size_R_3 | 20 | PRODUCT Size | 210 bp | Primer Start | 13451 | Primer End | 13660 |