Primers Available
Organism: Triacanthus biaculeatus mitochondrion
Repeat:(AACC)3
Start:8474 End:8485
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTCTGACTTGCCACTGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAACCCCTAGAGCCAAAGG | |||||||||||||
| Tm_F_1 | 59.891 | Size_F_1 | 20 | Tm_R_1 | 60.108 | Size_R_1 | 20 | PRODUCT Size | 157 bp | Primer Start | 8438 | Primer End | 8594 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTCTGACTTGCCACTGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCATTAACACGAACGCAGCT | |||||||||||||
| Tm_F_2 | 59.891 | Size_F_2 | 20 | Tm_R_2 | 59.834 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 8438 | Primer End | 8665 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAACGTAGCGGGACACAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAGGCACAGGGGTTCCTTC | |||||||||||||
| Tm_F_3 | 59.896 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 8300 | Primer End | 8528 |