Primers Available
Organism: Spratelloides gracilis mitochondrion
Repeat:(TTC)4
Start:6047 End:6058
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCAGACGCCTCTGTTTGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATAGGGTCTCCTCCTCCGG | |||||||||||||
| Tm_F_1 | 59.82 | Size_F_1 | 20 | Tm_R_1 | 59.811 | Size_R_1 | 20 | PRODUCT Size | 152 bp | Primer Start | 6004 | Primer End | 6155 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAGTACCAGACGCCTCTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATAGGGTCTCCTCCTCCGG | |||||||||||||
| Tm_F_2 | 60.251 | Size_F_2 | 20 | Tm_R_2 | 59.811 | Size_R_2 | 20 | PRODUCT Size | 157 bp | Primer Start | 5999 | Primer End | 6155 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCAGACGCCTCTGTTTGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCGGGGTCAAAGAAAGTTG | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 59.686 | Size_R_3 | 20 | PRODUCT Size | 134 bp | Primer Start | 6004 | Primer End | 6137 |