Primers Available
Organism: Spratelloides gracilis mitochondrion
Repeat:(CCGT)3
Start:3043 End:3054
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGAAGGGGCCCAATATTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCAAGGTGAGTGCTAAGGT | |||||||||||||
| Tm_F_1 | 60.106 | Size_F_1 | 20 | Tm_R_1 | 59.963 | Size_R_1 | 20 | PRODUCT Size | 146 bp | Primer Start | 2963 | Primer End | 3108 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTGTGCCCGTCCTCTTAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCAAGGTGAGTGCTAAGGT | |||||||||||||
| Tm_F_2 | 60.108 | Size_F_2 | 20 | Tm_R_2 | 59.963 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 2891 | Primer End | 3108 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGCCTATATTGTGCCCGTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCAAGGTGAGTGCTAAGGT | |||||||||||||
| Tm_F_3 | 59.892 | Size_F_3 | 20 | Tm_R_3 | 59.963 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 2883 | Primer End | 3108 |