Primers Available
Organism:Oncorhynchus masou 'Biwa' mitochondrion
Repeat:(T)13
Start:547 End:559
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCCCCTGAAAGCCGAATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACATGGGTCCTCTGGGGTC | |||||||||||||
| Tm_F_1 | 60.252 | Size_F_1 | 20 | Tm_R_1 | 60.326 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 446 | Primer End | 639 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCCCCTGAAAGCCGAATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATACATGGGTCCTCTGGGGT | |||||||||||||
| Tm_F_2 | 60.252 | Size_F_2 | 20 | Tm_R_2 | 59.653 | Size_R_2 | 20 | PRODUCT Size | 195 bp | Primer Start | 446 | Primer End | 640 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGACTCGTTACCCACCAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACATGGGTCCTCTGGGGTC | |||||||||||||
| Tm_F_3 | 59.684 | Size_F_3 | 20 | Tm_R_3 | 60.326 | Size_R_3 | 20 | PRODUCT Size | 146 bp | Primer Start | 494 | Primer End | 639 |