Primers Available
Organism: Astronotus ocellatus mitochondrion
Repeat:(CCTC)3
Start:5801 End:5812
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGAGCTCCAGACATAGCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTATTGAGAGACGGCTGGGG | |||||||||||||
| Tm_F_1 | 60.032 | Size_F_1 | 20 | Tm_R_1 | 59.895 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 5749 | Primer End | 6025 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGAGCTCCAGACATAGCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTATTGAGAGACGGCTGGG | |||||||||||||
| Tm_F_2 | 60.032 | Size_F_2 | 20 | Tm_R_2 | 59.895 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 5749 | Primer End | 6026 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCTTGAGCCGGAATAGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGATGAGGCCAAGAGGAGGA | |||||||||||||
| Tm_F_3 | 60.108 | Size_F_3 | 20 | Tm_R_3 | 59.957 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 5557 | Primer End | 5836 |