Primers Available
Organism: Cydistomyia duplonotata mitochondrion
Repeat:(TAA)4
Start:7319 End:7330
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTTGCCCCAATTCCAGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGCAGCCCCTACTCCTGTT | |||||||||||||
| Tm_F_1 | 58.34 | Size_F_1 | 20 | Tm_R_1 | 59.957 | Size_R_1 | 20 | PRODUCT Size | 168 bp | Primer Start | 7275 | Primer End | 7442 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTTGCCCCAATTCCAGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAATAGCAGCCCCTACTCC | |||||||||||||
| Tm_F_2 | 58.34 | Size_F_2 | 20 | Tm_R_2 | 60.251 | Size_R_2 | 20 | PRODUCT Size | 172 bp | Primer Start | 7275 | Primer End | 7446 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTTGCCCCAATTCCAGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATAGCAGCCCCTACTCCTGT | |||||||||||||
| Tm_F_3 | 58.34 | Size_F_3 | 20 | Tm_R_3 | 59.734 | Size_R_3 | 20 | PRODUCT Size | 169 bp | Primer Start | 7275 | Primer End | 7443 |