Primers Available
Organism: Oncorhynchus masou ishikawae mitochondrion
Repeat:(T)12
Start:547 End:558
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGACTCGTTACCCACCAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAAAAGCCGGGGAGATCTT | |||||||||||||
| Tm_F_1 | 59.684 | Size_F_1 | 20 | Tm_R_1 | 60.681 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 494 | Primer End | 738 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAAGCCGGGCGTTCTCTTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAAAAGCCGGGGAGATCTT | |||||||||||||
| Tm_F_2 | 60.462 | Size_F_2 | 20 | Tm_R_2 | 60.681 | Size_R_2 | 20 | PRODUCT Size | 229 bp | Primer Start | 510 | Primer End | 738 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACTCGTTACCCACCAAGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAAAAGCCGGGGAGATCTT | |||||||||||||
| Tm_F_3 | 60.673 | Size_F_3 | 20 | Tm_R_3 | 60.681 | Size_R_3 | 20 | PRODUCT Size | 243 bp | Primer Start | 496 | Primer End | 738 |