Primers Available
Organism: Pseudaspius leptocephalus mitochondrion
Repeat:(CTA)4
Start:5813 End:5824
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTGGCTACTACCACCGTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGCTGTCACAAGTACGGAT | |||||||||||||
| Tm_F_1 | 59.961 | Size_F_1 | 20 | Tm_R_1 | 60.109 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 5789 | Primer End | 6061 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTGGCTACTACCACCGTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTATTGGGAGATGGCTGGGG | |||||||||||||
| Tm_F_2 | 59.961 | Size_F_2 | 20 | Tm_R_2 | 59.889 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 5789 | Primer End | 6021 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTGGCTACTACCACCGTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTATTGGGAGATGGCTGGG | |||||||||||||
| Tm_F_3 | 59.961 | Size_F_3 | 20 | Tm_R_3 | 59.889 | Size_R_3 | 20 | PRODUCT Size | 234 bp | Primer Start | 5789 | Primer End | 6022 |