Primers Available
Organism: Misgurnus nikolskyi mitochondrion
Repeat:(TA)11
Start:16451 End:16472
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCCGAAACCAAGGAAGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTCCTGATTTTCGGGTTTTTGT | |||||||||||||
| Tm_F_1 | 59.89 | Size_F_1 | 20 | Tm_R_1 | 59.042 | Size_R_1 | 23 | PRODUCT Size | 152 bp | Primer Start | 16371 | Primer End | 16522 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGTTTTTGAGCGGCAAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTCCTGATTTTCGGGTTTTTGT | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.042 | Size_R_2 | 23 | PRODUCT Size | 216 bp | Primer Start | 16307 | Primer End | 16522 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCGAAACCAAGGAAGACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTCCTGATTTTCGGGTTTTTGT | |||||||||||||
| Tm_F_3 | 59.599 | Size_F_3 | 20 | Tm_R_3 | 59.042 | Size_R_3 | 23 | PRODUCT Size | 151 bp | Primer Start | 16372 | Primer End | 16522 |