Primers Available
Organism: Misgurnus nikolskyi mitochondrion
Repeat:(AACTT)3
Start:13574 End:13588
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGGCCGCCCTAACCAATAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCCAGCGGTTTCAAATCAT | |||||||||||||
| Tm_F_1 | 60.107 | Size_F_1 | 20 | Tm_R_1 | 59.468 | Size_R_1 | 20 | PRODUCT Size | 181 bp | Primer Start | 13464 | Primer End | 13644 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTCAAGCTAGCAGCCCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCCAGCGGTTTCAAATCAT | |||||||||||||
| Tm_F_2 | 59.749 | Size_F_2 | 20 | Tm_R_2 | 59.468 | Size_R_2 | 20 | PRODUCT Size | 237 bp | Primer Start | 13408 | Primer End | 13644 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTTCTCAAGCTAGCAGCCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCCAGCGGTTTCAAATCAT | |||||||||||||
| Tm_F_3 | 59.748 | Size_F_3 | 20 | Tm_R_3 | 59.468 | Size_R_3 | 20 | PRODUCT Size | 238 bp | Primer Start | 13407 | Primer End | 13644 |