Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(TCCT)3
Start:43301 End:43312
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCTTAGTGGATCGGCTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGCTTTCTACATAGGGATCGT | |||||||||||||
| Tm_F_1 | 59.738 | Size_F_1 | 20 | Tm_R_1 | 59.367 | Size_R_1 | 22 | PRODUCT Size | 269 bp | Primer Start | 43094 | Primer End | 43362 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCCAGTCGCTCACTAATTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCCGTGATCTGTCATTACG | |||||||||||||
| Tm_F_2 | 59.1 | Size_F_2 | 21 | Tm_R_2 | 58.791 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 43185 | Primer End | 43451 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCAGTCGCTCACTAATTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGATCTGTCATTACGTGCG | |||||||||||||
| Tm_F_3 | 59.1 | Size_F_3 | 21 | Tm_R_3 | 59.747 | Size_R_3 | 21 | PRODUCT Size | 264 bp | Primer Start | 43185 | Primer End | 43448 |