Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(T)13
Start:64481 End:64493
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAATACAGGCGTGGGGATCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTCTCCAAAACCCGATGT | |||||||||||||
| Tm_F_1 | 59.82 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 196 bp | Primer Start | 64439 | Primer End | 64634 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAATACAGGCGTGGGGATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTAGAACGCGGGTCTCCAAA | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.038 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 64439 | Primer End | 64643 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATACAGGCGTGGGGATCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTCTCCAAAACCCGATGT | |||||||||||||
| Tm_F_3 | 59.53 | Size_F_3 | 20 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 195 bp | Primer Start | 64440 | Primer End | 64634 |