Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(AT)7
Start:56573 End:56586
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTTGACAAATCGAGATTCGTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGCTATTCACAGCTACCACC | |||||||||||||
| Tm_F_1 | 58.189 | Size_F_1 | 23 | Tm_R_1 | 59.79 | Size_R_1 | 21 | PRODUCT Size | 268 bp | Primer Start | 56533 | Primer End | 56800 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGACTTGACAAATCGAGATTCGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGCTATTCACAGCTACCACC | |||||||||||||
| Tm_F_2 | 58.189 | Size_F_2 | 23 | Tm_R_2 | 59.79 | Size_R_2 | 21 | PRODUCT Size | 270 bp | Primer Start | 56531 | Primer End | 56800 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAGACTTGACAAATCGAGATTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGCTATTCACAGCTACCACC | |||||||||||||
| Tm_F_3 | 59.141 | Size_F_3 | 24 | Tm_R_3 | 59.79 | Size_R_3 | 21 | PRODUCT Size | 272 bp | Primer Start | 56529 | Primer End | 56800 |