Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(AGAA)3
Start:68964 End:68975
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTTGGAACACCAATGGGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCATTCGAATCACCCATTCCT | |||||||||||||
| Tm_F_1 | 59.584 | Size_F_1 | 21 | Tm_R_1 | 60.092 | Size_R_1 | 22 | PRODUCT Size | 263 bp | Primer Start | 68878 | Primer End | 69140 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTGGAACACCAATGGGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGCTACCCCTCTTTGTTTAGC | |||||||||||||
| Tm_F_2 | 58.5 | Size_F_2 | 20 | Tm_R_2 | 58.992 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 68879 | Primer End | 69158 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTTTTGGAACACCAATGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCATTCGAATCACCCATTCCT | |||||||||||||
| Tm_F_3 | 59.584 | Size_F_3 | 21 | Tm_R_3 | 60.092 | Size_R_3 | 22 | PRODUCT Size | 264 bp | Primer Start | 68877 | Primer End | 69140 |