Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(AAT)4
Start:64188 End:64199
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGTTTTCTACTGGCTGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCACGAATCTCAATGACCAAGA | |||||||||||||
| Tm_F_1 | 59.315 | Size_F_1 | 20 | Tm_R_1 | 59.739 | Size_R_1 | 23 | PRODUCT Size | 190 bp | Primer Start | 64105 | Primer End | 64294 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGGTTTTCTACTGGCTGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCACGAATCTCAATGACCAAGA | |||||||||||||
| Tm_F_2 | 59.315 | Size_F_2 | 20 | Tm_R_2 | 58.401 | Size_R_2 | 22 | PRODUCT Size | 189 bp | Primer Start | 64105 | Primer End | 64293 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGGTTTTCTACTGGCTGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCACGAATCTCAATGACCAAG | |||||||||||||
| Tm_F_3 | 59.315 | Size_F_3 | 20 | Tm_R_3 | 58.401 | Size_R_3 | 22 | PRODUCT Size | 190 bp | Primer Start | 64105 | Primer End | 64294 |