Primers Available
Organism: Hordeum vulgare subsp. vulgare chloroplast
Repeat:(AAGA)3
Start:72365 End:72376
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTTGAGACTAAATCATCGTGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGGGTCGATTTGCCTTGAA | |||||||||||||
| Tm_F_1 | 59.998 | Size_F_1 | 23 | Tm_R_1 | 59.964 | Size_R_1 | 20 | PRODUCT Size | 263 bp | Primer Start | 72203 | Primer End | 72465 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTTGCCTTTTGTTTAGCTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGGGTCGATTTGCCTTGAA | |||||||||||||
| Tm_F_2 | 59.931 | Size_F_2 | 23 | Tm_R_2 | 59.964 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 72186 | Primer End | 72465 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTGAGACTAAATCATCGTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATCAGCCACTGAATCGGGT | |||||||||||||
| Tm_F_3 | 59.998 | Size_F_3 | 23 | Tm_R_3 | 60.108 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 72203 | Primer End | 72479 |