Primers Available
Organism:Sorghum bicolor mitochondrion
Repeat:(TCTT)3
Start:174045 End:174056
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTCTTGCAGCGATACCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGATTCATTCTTTTTCGCA | |||||||||||||
| Tm_F_1 | 59.723 | Size_F_1 | 20 | Tm_R_1 | 60.016 | Size_R_1 | 20 | PRODUCT Size | 200 bp | Primer Start | 174015 | Primer End | 174214 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACATAGGCTCCTGGGTGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCGGCGCACTCTCTATTTT | |||||||||||||
| Tm_F_2 | 59.679 | Size_F_2 | 20 | Tm_R_2 | 59.982 | Size_R_2 | 20 | PRODUCT Size | 154 bp | Primer Start | 173929 | Primer End | 174082 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTCTTGCAGCGATACCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCAGGATGTGATCGGGATT | |||||||||||||
| Tm_F_3 | 59.723 | Size_F_3 | 20 | Tm_R_3 | 59.886 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 174015 | Primer End | 174271 |