Primers Available
Organism:Sorghum bicolor mitochondrion
Repeat:(TAGC)3
Start:229872 End:229883
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGAGCCCAAGATAAAGGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTACGGTTACCAACTCGCC | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 59.996 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 229703 | Primer End | 229964 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGGCCTTAGTGGTATTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTACGGTTACCAACTCGCC | |||||||||||||
| Tm_F_2 | 59.953 | Size_F_2 | 20 | Tm_R_2 | 59.996 | Size_R_2 | 20 | PRODUCT Size | 157 bp | Primer Start | 229808 | Primer End | 229964 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCCAGTTATCGGGACTTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTACGGTTACCAACTCGCC | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.996 | Size_R_3 | 20 | PRODUCT Size | 188 bp | Primer Start | 229777 | Primer End | 229964 |