Primers Available
Organism:Sorghum bicolor mitochondrion
Repeat:(T)11acaacttcgacttctgatagttttgattctatcagaaggaagaaggaaggggttatcccatgattaactctgcgatttttcacgctcgaacattgtctta(T)11
Start:291271 End:291392
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACCCCTTAGTACCTGGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTACCGAGCAAAGGCAAAA | |||||||||||||
| Tm_F_1 | 60.183 | Size_F_1 | 20 | Tm_R_1 | 59.883 | Size_R_1 | 20 | PRODUCT Size | 224 bp | Primer Start | 291220 | Primer End | 291443 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCTGGGGGATTCACTTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTACCGAGCAAAGGCAAAA | |||||||||||||
| Tm_F_2 | 59.755 | Size_F_2 | 20 | Tm_R_2 | 59.883 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 291248 | Primer End | 291443 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCAGATGATGAGGGGGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTACCGAGCAAAGGCAAAA | |||||||||||||
| Tm_F_3 | 59.694 | Size_F_3 | 20 | Tm_R_3 | 59.883 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 291195 | Primer End | 291443 |