Primers Available
Organism:Sorghum bicolor mitochondrion
Repeat:(ACT)4
Start:450687 End:450698
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACCGTCAAGATCGCAGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATAACATGTCAAGGGAGGCG | |||||||||||||
| Tm_F_1 | 60.263 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 196 bp | Primer Start | 450594 | Primer End | 450789 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGATCGCAGTTCACAAAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATAACATGTCAAGGGAGGCG | |||||||||||||
| Tm_F_2 | 59.596 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 188 bp | Primer Start | 450602 | Primer End | 450789 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGAGTCCGCCAGGAAATAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTCGAACTGACGAGGAGAG | |||||||||||||
| Tm_F_3 | 60.206 | Size_F_3 | 20 | Tm_R_3 | 60.284 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 450452 | Primer End | 450730 |