Primers Available
Organism:Nephroselmis olivacea mitochondrion
Repeat:(T)10(A)12
Start:25485 End:25506
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATTTCTACGGGCAAAGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTGGAGCCCAGACTTTCCT | |||||||||||||
| Tm_F_1 | 60.305 | Size_F_1 | 20 | Tm_R_1 | 60.074 | Size_R_1 | 20 | PRODUCT Size | 201 bp | Primer Start | 25397 | Primer End | 25597 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTATCCTCTGCTTGGAAGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTGGAGCCCAGACTTTCCT | |||||||||||||
| Tm_F_2 | 59.41 | Size_F_2 | 20 | Tm_R_2 | 60.074 | Size_R_2 | 20 | PRODUCT Size | 171 bp | Primer Start | 25427 | Primer End | 25597 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCTTATCCTCTGCTTGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTGGAGCCCAGACTTTCCT | |||||||||||||
| Tm_F_3 | 59.264 | Size_F_3 | 20 | Tm_R_3 | 60.074 | Size_R_3 | 20 | PRODUCT Size | 174 bp | Primer Start | 25424 | Primer End | 25597 |