Primers Available
Organism:Nephroselmis olivacea mitochondrion
Repeat:(GAT)4
Start:18423 End:18434
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACTTCGGACGTCGAGTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAATCCGATGGGAACACGTA | |||||||||||||
| Tm_F_1 | 60.291 | Size_F_1 | 20 | Tm_R_1 | 60.192 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 18235 | Primer End | 18511 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCGACGCCTCTGTAGGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAATCCGATGGGAACACGTA | |||||||||||||
| Tm_F_2 | 59.454 | Size_F_2 | 20 | Tm_R_2 | 60.192 | Size_R_2 | 20 | PRODUCT Size | 146 bp | Primer Start | 18366 | Primer End | 18511 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCGACGCCTCTGTAGGAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTTGCGTTAATGACTCCGC | |||||||||||||
| Tm_F_3 | 59.454 | Size_F_3 | 20 | Tm_R_3 | 60.278 | Size_R_3 | 20 | PRODUCT Size | 170 bp | Primer Start | 18366 | Primer End | 18535 |