Primers Available
Organism: Zygnema circumcarinatum chloroplast
Repeat:(TTGT)4
Start:47065 End:47080
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACACCACTTGAAATATGAACACCAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTGCAGCTGGTTTCGAAAA | |||||||||||||
| Tm_F_1 | 59.99 | Size_F_1 | 25 | Tm_R_1 | 59.969 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 46939 | Primer End | 47173 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACACCACTTGAAATATGAACACCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTCATTTGTGGCATGCAGG | |||||||||||||
| Tm_F_2 | 59.99 | Size_F_2 | 25 | Tm_R_2 | 60.109 | Size_R_2 | 20 | PRODUCT Size | 268 bp | Primer Start | 46939 | Primer End | 47206 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACACCACTTGAAATATGAACACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTGCAGCTGGTTTCGAAAA | |||||||||||||
| Tm_F_3 | 58.863 | Size_F_3 | 24 | Tm_R_3 | 59.969 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 46939 | Primer End | 47173 |