Primers Available
Organism: Zygnema circumcarinatum chloroplast
Repeat:(AATA)6aattaataaattaacaaaatact(TAATA)7
Start:143404 End:143485
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTCCACGAACCTACCCTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGAACTTTCTTTATAGCTTCCT | |||||||||||||
| Tm_F_1 | 59.653 | Size_F_1 | 21 | Tm_R_1 | 57.949 | Size_R_1 | 24 | PRODUCT Size | 207 bp | Primer Start | 143337 | Primer End | 143543 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTCCACGAACCTACCCTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCGAACTTTCTTTATAGCTTCCT | |||||||||||||
| Tm_F_2 | 58.573 | Size_F_2 | 20 | Tm_R_2 | 57.949 | Size_R_2 | 24 | PRODUCT Size | 206 bp | Primer Start | 143338 | Primer End | 143543 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTTCCACGAACCTACCCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCGAACTTTCTTTATAGCTTCCT | |||||||||||||
| Tm_F_3 | 59.378 | Size_F_3 | 21 | Tm_R_3 | 57.949 | Size_R_3 | 24 | PRODUCT Size | 208 bp | Primer Start | 143336 | Primer End | 143543 |