Primers Available
Organism: Zygnema circumcarinatum chloroplast
Repeat:(AAAT)3
Start:109995 End:110006
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCGAGTATGTTTCTTTTCTGTTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCTCGCCGTATGAAAGCGA | |||||||||||||
| Tm_F_1 | 57.27 | Size_F_1 | 25 | Tm_R_1 | 60.248 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 109893 | Primer End | 110109 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCGAGTATGTTTCTTTTCTGTTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAACCAATGACTCTCGCCG | |||||||||||||
| Tm_F_2 | 57.27 | Size_F_2 | 25 | Tm_R_2 | 60.39 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 109893 | Primer End | 110120 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCGAGTATGTTTCTTTTCTGTTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAACCAATGACTCTCGCCGT | |||||||||||||
| Tm_F_3 | 57.27 | Size_F_3 | 25 | Tm_R_3 | 60.39 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 109893 | Primer End | 110119 |