Primers Available
Organism: Percina macrolepida mitochondrion
Repeat:(AACC)3
Start:8462 End:8473
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCGCAGTCCCCCTTTGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACAAATGCAGCTGTGGCA | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.968 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 8416 | Primer End | 8647 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTTAGGGCTCGCAGTCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAACAAATGCAGCTGTGGCA | |||||||||||||
| Tm_F_2 | 60.107 | Size_F_2 | 20 | Tm_R_2 | 59.968 | Size_R_2 | 20 | PRODUCT Size | 241 bp | Primer Start | 8407 | Primer End | 8647 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCGCAGTCCCCCTTTGATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAACAAATGCAGCTGTGGC | |||||||||||||
| Tm_F_3 | 60.035 | Size_F_3 | 20 | Tm_R_3 | 59.684 | Size_R_3 | 20 | PRODUCT Size | 233 bp | Primer Start | 8416 | Primer End | 8648 |