Primers Available
Organism: Helicosporidium sp. ex Simulium jonesii plastid
Repeat:(AAAT)3
Start:27178 End:27189
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCAACCACAGTTTAGAATTTACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTTCCACCAAATTTGCGTCC | |||||||||||||
| Tm_F_1 | 57.047 | Size_F_1 | 24 | Tm_R_1 | 59.66 | Size_R_1 | 21 | PRODUCT Size | 252 bp | Primer Start | 27044 | Primer End | 27295 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCAACCACAGTTTAGAATTTACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTCCACCAAATTTGCGTCCT | |||||||||||||
| Tm_F_2 | 57.047 | Size_F_2 | 24 | Tm_R_2 | 59.387 | Size_R_2 | 21 | PRODUCT Size | 251 bp | Primer Start | 27044 | Primer End | 27294 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCAACCACAGTTTAGAATTTACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCCACCAAATTTGCGTCCT | |||||||||||||
| Tm_F_3 | 57.047 | Size_F_3 | 24 | Tm_R_3 | 58.301 | Size_R_3 | 20 | PRODUCT Size | 250 bp | Primer Start | 27044 | Primer End | 27293 |