Primers Available
Organism: Hynobius leechii mitochondrion
Repeat:(TAA)4(ATTT)3
Start:4529 End:4552
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTGGGGGATGGGGAGGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTCCGCCCAGGGATATAAGTG | |||||||||||||
| Tm_F_1 | 60.331 | Size_F_1 | 20 | Tm_R_1 | 59.998 | Size_R_1 | 21 | PRODUCT Size | 271 bp | Primer Start | 4419 | Primer End | 4689 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTGGGGGATGGGGAGGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTCCGCCCAGGGATATAAGTG | |||||||||||||
| Tm_F_2 | 60.331 | Size_F_2 | 20 | Tm_R_2 | 59.998 | Size_R_2 | 21 | PRODUCT Size | 272 bp | Primer Start | 4418 | Primer End | 4689 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGGGATGGGGAGGATTAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCCGCCCAGGGATATAAGTG | |||||||||||||
| Tm_F_3 | 59.648 | Size_F_3 | 20 | Tm_R_3 | 59.998 | Size_R_3 | 21 | PRODUCT Size | 269 bp | Primer Start | 4421 | Primer End | 4689 |