Primers Available
Organism: Hynobius amjiensis mitochondrion
Repeat:(TTAA)3
Start:1115 End:1126
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCGTCACCCTCCTCAAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCGCTAATGAGTTTTTCTATCTCC | |||||||||||||
| Tm_F_1 | 59.89 | Size_F_1 | 20 | Tm_R_1 | 59.476 | Size_R_1 | 25 | PRODUCT Size | 280 bp | Primer Start | 898 | Primer End | 1177 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGTCACCCTCCTCAAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGCTAATGAGTTTTTCTATCTCCT | |||||||||||||
| Tm_F_2 | 59.89 | Size_F_2 | 20 | Tm_R_2 | 59.244 | Size_R_2 | 25 | PRODUCT Size | 279 bp | Primer Start | 898 | Primer End | 1176 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCGTCACCCTCCTCAAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGCTAATGAGTTTTTCTATCTCC | |||||||||||||
| Tm_F_3 | 59.89 | Size_F_3 | 20 | Tm_R_3 | 58.02 | Size_R_3 | 24 | PRODUCT Size | 279 bp | Primer Start | 898 | Primer End | 1176 |