Primers Available
Organism: Discosoma sp. CASIZ 168916 mitochondrion
Repeat:(TTA)4
Start:8585 End:8596
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGTTGGATCGGATGCCACTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCAAAAATCTGTCTCTTGGCA | |||||||||||||
| Tm_F_1 | 59.896 | Size_F_1 | 20 | Tm_R_1 | 59.107 | Size_R_1 | 22 | PRODUCT Size | 278 bp | Primer Start | 8534 | Primer End | 8811 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTTGGATCGGATGCCACTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCCAAAAATCTGTCTCTTGGC | |||||||||||||
| Tm_F_2 | 59.896 | Size_F_2 | 20 | Tm_R_2 | 59.107 | Size_R_2 | 22 | PRODUCT Size | 279 bp | Primer Start | 8534 | Primer End | 8812 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGCACAGGATGAACGGTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCATAGTTATACCCCCAGCT | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 58.008 | Size_R_3 | 21 | PRODUCT Size | 261 bp | Primer Start | 8370 | Primer End | 8630 |