Primers Available
Organism: Discosoma sp. CASIZ 168916 mitochondrion
Repeat:(TTA)4
Start:11958 End:11969
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGAACCTTGACACTTGGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCCACAACCTCATCCATCA | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 60.034 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 11867 | Primer End | 12138 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGACACTTGGGTTTGCAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCCACAACCTCATCCATCA | |||||||||||||
| Tm_F_2 | 59.743 | Size_F_2 | 20 | Tm_R_2 | 60.034 | Size_R_2 | 20 | PRODUCT Size | 264 bp | Primer Start | 11875 | Primer End | 12138 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACACTTGGGTTTGCAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCAAAACAGGGCCCACAAC | |||||||||||||
| Tm_F_3 | 59.743 | Size_F_3 | 20 | Tm_R_3 | 59.896 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 11875 | Primer End | 12149 |