Primers Available
Organism:Paracoccidioides brasiliensis mitochondrion
Repeat:(AAT)4
Start:28259 End:28270
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAATTCCTTTTCTTCCTAAATGGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTGAAAAGGCCTATAAGAAAGAAT | |||||||||||||
| Tm_F_1 | 60.083 | Size_F_1 | 25 | Tm_R_1 | 57.42 | Size_R_1 | 25 | PRODUCT Size | 270 bp | Primer Start | 28145 | Primer End | 28414 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAATTCCTTTTCTTCCTAAATGGATA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTGAAAAGGCCTATAAGAAAGAA | |||||||||||||
| Tm_F_2 | 60.089 | Size_F_2 | 26 | Tm_R_2 | 57.073 | Size_R_2 | 24 | PRODUCT Size | 270 bp | Primer Start | 28145 | Primer End | 28414 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCCTTTTCTTCCTAAATGGATAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTGAAAAGGCCTATAAGAAAGAA | |||||||||||||
| Tm_F_3 | 57.555 | Size_F_3 | 24 | Tm_R_3 | 57.073 | Size_R_3 | 24 | PRODUCT Size | 267 bp | Primer Start | 28148 | Primer End | 28414 |