Primers Available
Organism: Anisakis simplex mitochondrion
Repeat:(TTTTA)3
Start:11680 End:11694
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCATACACGAGATTTGGAGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACAACCTCTTCATTGTAAGTGAAGT | |||||||||||||
| Tm_F_1 | 60.093 | Size_F_1 | 22 | Tm_R_1 | 58.643 | Size_R_1 | 25 | PRODUCT Size | 149 bp | Primer Start | 11574 | Primer End | 11722 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCATACACGAGATTTGGAGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTCATAACACACCTCAAAATAGCT | |||||||||||||
| Tm_F_2 | 60.093 | Size_F_2 | 22 | Tm_R_2 | 58.524 | Size_R_2 | 25 | PRODUCT Size | 205 bp | Primer Start | 11574 | Primer End | 11778 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCATACACGAGATTTGGAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAACCTCTTCATTGTAAGTGAAGT | |||||||||||||
| Tm_F_3 | 58.72 | Size_F_3 | 21 | Tm_R_3 | 58.643 | Size_R_3 | 25 | PRODUCT Size | 149 bp | Primer Start | 11574 | Primer End | 11722 |