Primers Available
Organism: Anisakis simplex mitochondrion
Repeat:(TTTG)3
Start:1774 End:1785
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGCTACCCGTTTGTTTTTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCCACACCGACCTTTAACA | |||||||||||||
| Tm_F_1 | 59.59 | Size_F_1 | 21 | Tm_R_1 | 60.881 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 1651 | Primer End | 1916 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGCTACCCGTTTGTTTTTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCATAAGAGCCCAAACCCT | |||||||||||||
| Tm_F_2 | 59.59 | Size_F_2 | 21 | Tm_R_2 | 58.997 | Size_R_2 | 20 | PRODUCT Size | 158 bp | Primer Start | 1651 | Primer End | 1808 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGCTACCCGTTTGTTTTTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAAAAATGCAGAGGCGCCAC | |||||||||||||
| Tm_F_3 | 59.59 | Size_F_3 | 21 | Tm_R_3 | 61.01 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 1651 | Primer End | 1930 |