Primers Available
Organism: Anisakis simplex mitochondrion
Repeat:(TGTTT)3
Start:4027 End:4041
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGGGTTTACTAGTTCTTTGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCAGGAACAAGAGCAGCAT | |||||||||||||
| Tm_F_1 | 57.608 | Size_F_1 | 23 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 3973 | Primer End | 4224 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGGGTTTACTAGTTCTTTGGTAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCAGGAACAAGAGCAGCAT | |||||||||||||
| Tm_F_2 | 59.392 | Size_F_2 | 25 | Tm_R_2 | 59.962 | Size_R_2 | 20 | PRODUCT Size | 251 bp | Primer Start | 3974 | Primer End | 4224 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTGGGGTTTACTAGTTCTTTGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCAGGAACAAGAGCAGCAT | |||||||||||||
| Tm_F_3 | 58.231 | Size_F_3 | 24 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 253 bp | Primer Start | 3972 | Primer End | 4224 |