Primers Available
Organism: Anisakis simplex mitochondrion
Repeat:(ATT)4
Start:10187 End:10198
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTGCTTCTAGTAACTATGAGTGTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGCCCCTCTGCACCTATCC | |||||||||||||
| Tm_F_1 | 57.444 | Size_F_1 | 27 | Tm_R_1 | 60.472 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 10118 | Primer End | 10344 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTGCTTCTAGTAACTATGAGTGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAGCCCCTCTGCACCTATCC | |||||||||||||
| Tm_F_2 | 57.444 | Size_F_2 | 27 | Tm_R_2 | 60.472 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 10117 | Primer End | 10344 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTTTGCTTCTAGTAACTATGAGTGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAGCCCCTCTGCACCTATCC | |||||||||||||
| Tm_F_3 | 57.274 | Size_F_3 | 27 | Tm_R_3 | 60.472 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 10116 | Primer End | 10344 |