Primers Available
Organism: Sepia officinalis mitochondrion
Repeat:(AT)13(GT)7
Start:9495 End:9534
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCCTCCATTGCCCGTTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCATTCCGGCGCAGTATCA | |||||||||||||
| Tm_F_1 | 61.191 | Size_F_1 | 20 | Tm_R_1 | 59.823 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 9362 | Primer End | 9623 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAAAGGCCCCTCCATTGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCATTCCGGCGCAGTATCA | |||||||||||||
| Tm_F_2 | 61.201 | Size_F_2 | 20 | Tm_R_2 | 59.823 | Size_R_2 | 20 | PRODUCT Size | 269 bp | Primer Start | 9355 | Primer End | 9623 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCTCCATTGCCCGTTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATTCCGGCGCAGTATCAG | |||||||||||||
| Tm_F_3 | 61.191 | Size_F_3 | 20 | Tm_R_3 | 60.249 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 9362 | Primer End | 9622 |