Primers Available
Organism:Dictyostelium citrinum mitochondrion
Repeat:(AGA)5
Start:5335 End:5349
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTCAAAAATTGGCGTTTCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTCATCCATCGACGTTTT | |||||||||||||
| Tm_F_1 | 60.11 | Size_F_1 | 20 | Tm_R_1 | 58.976 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 5158 | Primer End | 5429 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTCAAAAATTGGCGTTTCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTATCCCACGCATCTAATCG | |||||||||||||
| Tm_F_2 | 60.11 | Size_F_2 | 20 | Tm_R_2 | 60.067 | Size_R_2 | 21 | PRODUCT Size | 240 bp | Primer Start | 5158 | Primer End | 5397 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAGTGTTTGATGTGGCTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTTCATCCATCGACGTTTT | |||||||||||||
| Tm_F_3 | 59.758 | Size_F_3 | 20 | Tm_R_3 | 58.976 | Size_R_3 | 20 | PRODUCT Size | 167 bp | Primer Start | 5263 | Primer End | 5429 |